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Tuesday, November 24, 2020 | History

2 edition of site of action of radio-lethal compounds on Micrococcus radiodurans found in the catalog.

site of action of radio-lethal compounds on Micrococcus radiodurans

Manes, Jasper Damon Jr.

site of action of radio-lethal compounds on Micrococcus radiodurans

  • 71 Want to read
  • 12 Currently reading

Published .
Written in English

    Subjects:
  • Bacteria -- Effect of radiation on.,
  • Radiation -- Physiological effect.

  • Edition Notes

    Statementby Jasper Damon Manes, Jr.
    The Physical Object
    Pagination[9], 55 leaves, bound :
    Number of Pages55
    ID Numbers
    Open LibraryOL14254753M

    Resistance of Micrococcus radiodurans K. L. KRABBENHOFT,' A. W. ANDERSON, AND P. R. ELLIKER DepartmentofMicrobiology, OregonState University, Corvallis, Oregon Received for publication 29 August ABSTRACT The addition of NZ-case (a tryptic digest of casein) to a growth medium (PC) consisting of tryptone, glucose, and yeast extract caused a. A simplified technique permitting the pipetting of raw puréed meats for quantitative bacteriological study is described for use in determining survival of these non-sporing bacteria, which are exceptionally resistant to radiation. Survival curves, using gamma radiation as the sterilizing agent, were determined in raw beef with four strains of Micrococcus radiodurans.


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site of action of radio-lethal compounds on Micrococcus radiodurans by Manes, Jasper Damon Jr. Download PDF EPUB FB2

The site of action of radio-lethal compounds on Micrococcus radiodurans Public Deposited. Analytics. Downloadable Content Iodoacetic acid and diphenylamine were used in this study to elucidate the mechanisms of resistance of Micrococcus radiodurans to radiation.

This organism, a gram positive, non-spore forming, salmon-pink, tetracoccus has Author: Jasper Damon Manes. THE SITE OF ACTION OF RADIO - LETHAL COMPOUNDS ON MICROCOCCUS RADIODURANS INTRODUCTION The objective of this study was to identify the molecules respon- sible for the extreme radiation resistance of Micrococcus radiodurans.

Because of. The site of action of radio-lethal compounds on Micrococcus radiodurans. Abstract. Graduation date: Iodoacetic acid and diphenylamine were used in this study to\ud elucidate the mechanisms of resistance of Micrococcus radiodurans\ud to radiation.

This organism, a gram positive, non-spore forming,\ud salmon-pink, tetracoccus has a. Abstract. An ecological study of Micrococcus radiodurans indicated that microorganisms possessing the same morphological and radiation-resistance characteristics as that organism could be isolated from ground beef and from pork sausage.

Further studies showed that such organisms also could be isolated from beef hides and from water from a creek adjacent to the packing plant from which the meat Cited by: Abstract. Micrococcus radiodurans, a gram-positive, nonsporing, red-pigmented bacterium, is the type species of a small group of bacteria, the members of which are characterized by extreme resistance to both the lethal and mutagenic effects of ionizing and ultraviolet (UV) show no loss of viability up to doses of krad or Jm-2 of ionizing or UV radiation, respectively Cited by: Micrococcus radiodurans because of its superficial morphological similarity to members of the genus Micrococcus.

However, research on M. radiodurans over the next 30 years resulted in reclassification of this species and its closest relatives into a distinct phylum within the domain Bacteria2–7. The genus. Micrococcus radiodurans Raj et al. Verified By Whole-genome Sequencing Strain Designations R1 (smooth) [CCUGCIPDSMHAMBIIFOLMGUWOVKM B].

Mattingly and H.J.R. Copland, Sensitisation to radiation by loss of recombination ability in a temperature-sensitive DNA mutant of Micrococcus radiodurans held at its restrictive tem- perature, J.

Gen. Microbiol., 72 () 21 Moseley, B.E.B. and H.J.R. Copland, Isolation and properties of a recombination-deficient mutant of. A 5′-nucleotidase (5′-ribonucleotide phosphohydrolase, EC ) isolated from Micrococcus radiodurans appears to have properties more closely resembling some of the corresponding vertebrate enzymes than the reported bacterial enzymes.

The M. radiodurans enzyme is a strict nucleoside 5′-phosphomonoesterase with a pH optimum between 8 and 9. It is maximally. Deinococcus radiodurans was the first radioactive tolerant bacteria discovered by Arthur Anderson while performing the experiments to determine the sterility of canned foods using gamma radiation.

Deinococcus radiodurans 1. •one of the most radiation-resistant organisms known. •a polyextremophile •the world's toughest bacterium in The Guinness Book Of World Records •from the Ancient Greek δεινός (deinos) and κόκκος (kokkos) meaning "terrible grain/berry" and the Latin radius and durare, meaning "radiation surviving".

•formerly called Micrococcus radiodurans. Cite this article. ASHWOOD-SMITH, M., COPELAND, J. & WILCOCKSON, J. Response of Bacterial Spores and Micrococcus radiodurans to Ultraviolet Irradiation at Low. The cell envelope of Micrococcus radiodurans has been examined by Ta/W shadowing complemented by Ag decoration.

Particular attention was paid to the structure determination of the HPI layer, a hexagonally patterned monolayer of protein, and its association with the. We have called this mutant Micrococcus radiodurans rec The efficiency of recombination in this mutant, as measured by transformation, is less than % that of the wild type.

Site of action of radio-lethal compounds on Micrococcus radiodurans book. The field of radiobiology was built on the premise that radiation is dangerous because of its damaging effects on DNA, where only a few events, or even a single event, at the molecular level can inactivate cells (Hutchinson ).The discordance of modern radiation toxicity models with results spanning nearly 5 decades of research on the extremely radiation-resistant bacterium.

Deinococcus radiodurans is an extremophilic bacterium and one of the most radiation-resistant organisms known. It can survive cold, dehydration, vacuum, and acid, and therefore, is known as a polyextremophile and it has been listed as the world's toughest known bacterium in The Guinness Book Of World Records.

The Guinness Book of World Records says that Deinococcus radiodurans is the world’s most extremophile bacterium. As its name suggests, this bacterium can deal with radiation. It has even been found on the inside walls of nuclear reactors. The dual-luciferase reporter vector pRADN4 was constructed on the basis of pRADN2.

The D. radiodurans groE minimal promoter (Meima et al., ) was amplified by PCR from genomic DNA with primers PgroNF and resulting PCR fragment ( bp) was inserted into a pre-blunted NspV site of pRL-null (Promega) to yield plasmid y, a bp BamHI–PstI.

setlow jk, duggan de. the resistance of micrococcus radiodurans to ultraviolet radiation. ultraviolet-induced lesions in the cell's dna. biochim biophys acta. aug 12; – young et, 2nd, sinsheimer rl. a comparison of the initial actions of spleen deoxyribonuclease and pancreatic deoxyribonuclease.

Deinococcus radiodurans is a gram-positive, nonsporulating bacterium which usually grows in tetrad form. This organism is interesting because of its extreme resistance to DNA damage induced by ionizing radiation ().It exhibits detectable survival after irradiation of 15, Gy and grows continuously at 60 Gy/h ().The unusual radiation resistance of D.

radiodurans results from its ability to. Deinococcus radiodurans is extremely resistant to the lethal and mutagenic effects of ionizing-radiation and many other physical and chemical agents that damage DNA.

This resistance is known to be due to D. radiodurans' extremely proficient DNA repair processes. However, little is known about the precise mechanisms employed by this organism to achieve its efficient repair.

Deinococcus radiodurans was first classified as Micrococcus radiodurans, due to its similarities to that genus. However, after further research, it was later put into a phylum of its own [4].

“Deinos”, in Greek, means strange or unusual, which is a good description of this bacteria [7]. Sublethal â °rradiation of the bacterium Micrococcus radiodurans in aqueous suspension results in a loss of up to 6 percent of its cellular dry weight and 30 percent of its wet.

Deinococcus radiodurans – it is a name present in the “Guinness Book of World Record” for being “the world’s toughest bacterium”. Now, that you know that with such a complex name, there is a bacterium, you might be wondering how tough it is actually.

Well, the microbe is almost indestructible, bearing the capability of surviving at. Deinococcus radiodurans ("strange berry that withstands radiation", formerly called Micrococcus radiodurans) is an extremophilic bacterium, and is the most radioresistant organism known.

While a dose of 10 Gy is sufficient to kill a human, and a dose of 60 Gy is sufficient to kill all cells in a culture of E. coli, D. radiodurans is capable of withstanding an instantaneous dose of up to 5, Baumeister W, Kübler O.

Topographic study of the cell surface of micrococcus radiodurans. Proc Natl Acad Sci U S A. Nov; 75 (11)– [PMC free article] Carbonneau MA, Melin AM, Perromat A, Clerc M. The action of free radicals on Deinococcus radiodurans carotenoids.

Arch Biochem Biophys. Nov 15; (1)– Ecology of Micrococcus radiodurans. Appl. Microbiol. An ecological study of Micrococcus radiodurans indicated that microorganisms possessing the same morphological and radiation-resistance characteristics as that organism could be isolated from ground beef and from pork sausage.

Further studies. The polyextremophile, gram-positive bacterium Deinococcus radiodurans can withstand harsh conditions of real and simulated outer space environment, e.g., UV and ionizing radiation.

A long-term space exposure of D. radiodurans has been performed in Low Earth Orbit (LEO) in frames of the Tanpopo orbital mission aiming to investigate the possibility of interplanetary life transfer. Space. Deinococcus (Micrococcus) radiodurans R1 has extreme resistance to genotoxic chemicals, oxidative damage, high levels of ionizing and UV radiation, and desiccation (4, 7, 12, 19).It has been suggested that the extreme resistance to ionizing radiation is attributable to an effective DNA repair system and a special chromosome structure (8, 13, 14, 16–18).

Abstract. Various aspects of the repair of ultraviolet (UV) radiation-induced damage were compared in wild-type Micrococcus radiodurans and two UV-sensitive mutants. Unlike the wild type, the mutants are more sensitive to radiation at nm than at nm.

Transformation in Micrococcus radiodurans and the ultraviolet sensitivity of its transforming DNA. The complexity of the genome of Micrococcus radiodurans was determined to be ( +/- ) X 10(9) daltons by DNA renaturation kinetics.

of carbonyl compounds per mole of leucine oxidized is. Micrococcus radiodurans, irradiated aerobically in phosphate buffer, exhibits a phasic response to x-irradiation during its growth cycle. The culture progresses from a relatively sensitive state during the exponential portion of the growth cycle to a highly resistant state during the stationary phase.

A comparative study of σ factors of bacterial genomes by Kill et al. also showed that σ factors of D. radiodurans R1 were very divergent and, therefore, not detected by in silico studies (Kill et al., ). In silico search of the genome of D.

radiodurans R1 (Liu et al., ) for σ factors showed lack of a stress regulator (sig B). The radiation resistant bacteria Micrococcus radiophilus and M.

radioproteolyticus were studied by thin sectioning and freeze-etching techniques and the two species were found to be similar in the fine structure. The only significant difference was in the appearance of the surfaces of the cell walls in freeze-etched preparations.

Since the two species, together with M. radiodurans, possess a. Deinococcus radiodurans R1, has been recognised as a potential candidate for bioremediation process of radioactive wastes due to its surprisingly high radioresistance, however, it does not form. Introduction. Deinococcus (formally Micrococcus) radiodurans is able to withstand extraordinary levels of DNA damage.

This organism is able to restore its genome completely, without mutations or rearrangements, after sustaining as many as double-stranded breaks per chromosome (Kitayama & Matsuyama,Daly et al., ).In comparison, Escherichia coli can only withstand two to three.

AN ABSTRACT OF THE THESIS OF Kenneth Louis Krabbenhoft for the Ph. D, in Microbiology (Name) (Degree) (Major) Date thesis is presented T- Title SIGNIFICANCE OF PIGMENTS IN RADIATION RESISTANCE OF MICROCOCCUS RADIODURANS Abstract approved (Major professor) An ecological study of a microorganism similar to that iso- lated in and designated as Micrococcus radiodurans.

Introduction. Deinococcus radiodurans possesses extraordinary resistance to the lethal and mutagenic effects of ionizing and UV radiation and many other DNA damaging agents, which is thought to be effected by a highly proficient DNA repair capacity (Moseley, ; Battista, ).The most striking feature of D.

radiodurans is its capacity for repairing ionizing radiation‐induced DNA double. Dec. 14, Like a muscle-bound movie hero, it withstands attacks from acid baths, high and low temperatures, and even radiationin a science fiction sequel, it dispenses lifesaving medications and reshapes a planet for new settlers.

Right: D. radiodurans growing on a nutrient agar plate. The red color is due to carotenoid pigment. Deinococcus radiodurans.

Description and significance. Deinococcus radiodurans is a red-pigmented, non-motile, spherical bacterium that is µm in size. It is a resilient bacterium, in that it is resistant to radiation, UV light, hydrogen peroxide, and many other DNA damaging agents.Name and classification.

The name Deinococcus radiodurans derives from the Ancient Greek δεινός (deinos) and kokkos meaning "terrible grain" and the Latin radius and durare, meaning "radiation surviving".The species was formerly called Micrococcus a consequence of its hardiness, it has been nicknamed Conan the Bacterium.

[2]Initially, it was placed in the genus Micrococcus.Deinococcus radiodurans is a bacteria best known as one of the most radiation-resistant organisms known.

Its name basically means 'strange berry that withstands radiation', and the The Guinness Book of Records named it the “World‘s toughest bacterium”. It can tolerate radiation levels at times the levels that would kill a human and it was originally isolated in from a can of.